A sensitive, reproducible and rapid reverse phase high performance liquid chromatographic methods were developed and validated for the determination of Ziprasidone Hydrochloride Monohydrate (ZHM) in presence of its degradation products in pure form and pharmaceutical formulations. ACE-5 C18 column (150Ã?â??4.6mm, 5Ã?µ) was used with a mobile phase containing a mixture of Acetonitrile : Phosphate Buffer pH 3.2 in the ratio of (40:60). The flow rate was 1ml/min and effluents were monitored at 219 nm and eluted at 2.83 min. Calibration curve was plotted with a range from 10-50 Ã?µg/ml .The method was validated for the parameters like accuracy, precision, robustness and system suitability parameters. The precision, ruggedness and robustness values were also within the prescribed limits (< 1% for system precision and < 2% for other parameters). The LOD and LOQ was found to be 0.1282 Ã?µg/ml & 0.3885 Ã?µg/ml respectively.
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